Evaluation and validation of a duck IgY antibody-based immunoassay for high-sensitivity C-reactive protein: avian antibody application in clinical diagnostics.
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چکیده
1. Kovalevskaya G, Birken S, Kakuma T, Ozaki N, Sauer M, Lindheim S, et al. Differential expression of human chorionic gonadotropin (hCG) glycosylation isoforms in failing and continuing pregnancies: preliminary characterization of the hyperglycosylated hCG epitope. J Endocrinol 2002;172:497–506. 2. Elliott MM, Kardana A, Lustbader JW, Cole LA. Carbohydrate and peptide structure of the and -subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma. Endocrine 1997;7: 15–32. 3. Frendo JL, Vidaud M, Guibourdenche J, Luton D, Muller F, Bellet D, et al. Defect of villous cytotrophoblast differentiation in syncytiotrophoblast in Down’s syndrome. J Clin Endocrinol Metab 2000;85:3700–7. 4. Massin N, Frendo JL, Guibourdenche J, Luton D, Giovangrandi Y, Muller F, et al. Defect of syncytiotrophoblast formation and human chorionic gonadotropin expression in Down’s syndrome. Placenta 2001;22(Suppl A):S93–7. 5. Cole LA, Shahabi S, Oz UA, Bahado-Singh RO, Mahoney MJ. Hyperglycosylated human chorionic gonadotropin (invasive trophoblast antigen) immunoassay: a new basis for gestational Down syndrome screening. Clin Chem 1999;45:2109–19. 6. Kovalevskaya G, Genbacev O, Fisher S, Caceres E, O’Connor J. Trophoblast origin of hCG isoforms: cytotrophoblasts are the primary source of choriocarcinoma-like hCG. Mol Cell Endocrinol 2002;194:147–55. 7. Birken S, Krichevsky A, O’Connor J, Schlatterer J, Cole L, Kardana A, et al. Development and characterization of antibodies to a nicked and hyperglycosylated form of hCG from a choriocarcinoma patient. Endocrine 1999;10: 137–44. 8. Kovalevskaya G, Birken S, Kakuma T, O’Connor JF. Early pregnancy human chorionic gonadotropin (hCG) isoforms measured by an immunometric assay for choriocarcinoma-like hCG. J Endocrinol 1999;161:99–106. 9. Cole LA, Omrani A, Cermik D, Bahado-Singh RO, Mahoney MJ. Hyperglycosylated hCG, a potential alternative to hCG in Down syndrome screening. Prenat Diagn 1998;18:926–33. 10. Cole LA, Shahabi S, Oz UA, Rinne KM, Omrani A, Bahado-Singh RO, et al. Urinary screening tests for fetal Down syndrome: II. Hyperglycosylated hCG. Prenat Diagn 1999;19:351–9. 11. Bahado-Singh R, Oz U, Shahabi S, Omrani A, Mahoney M, Cole L. Urine hyperglycosylated hCG plus ultrasound biometry for detection of Down syndrome in the second trimester in a high-risk population. Obstet Gynecol 2000;95:889–94. 12. Khanlian SA, Smith HO, Cole LA. Persistent low levels of hCG: a premalignant gestational trophoblastic disease. Am J Obstet Gynecol;in press. 13. Diamandis EP, Christopoulos TK. The biotin-(strept)avidin system: principles and applications in biotechnology [Review]. Clin Chem 1991;37:625–36. 14. Weeks I, Beheshti I, McCapra F, Campbell AK, Woodhead JS. Acridinium esters as high-specific-activity labels in immunoassay. Clin Chem 1983;29: 1474–9. 15. O’Connor JF, Ellish N, Kakuma T, Schlatterer J, Kovalevskaya G. Differential urinary gonadotropin profiles in early pregnancy and early pregnancy loss. Prenat Diagn 1998;18:1232–40. 16. Butler SA, Khanlian SA, Cole LA. Detection of early pregnancy forms of human chorionic gonadotropin by home pregnancy test devices. Clin Chem 2001;47:2131–6.
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 49 5 شماره
صفحات -
تاریخ انتشار 2003